Effect of human umbilical cord mesenchymal stem cells on ovarian function in lupus mice

Background Patients with systemic lupus erythematosus (SLE) suffer from a high incidence of premature ovarian failure, which might be due to cyclophosphamide gonadal toxicity, immune abnormalities, or other reasons. This study aimed investigate whether the transplantation human umbilical cord mesenchymal stem cells (HUC-MSCs) can improve reserve function in mice. Methods We used MRL/lpr mice observe changes structure and secretory SLE. Lupus controls were injected HUC-MSCs at Weeks 12 16. detected serum concentrations sex hormones estradiol (E2), follicle-stimulating hormone (FSH), anti-Müllerian (AMH), using enzyme-linked immunosorbent assays. Hematoxylin eosin staining showed tissue enabled counting follicles. Hepatocyte growth factor (HGF) insulin-like 1 (IGF-1) expression was observed by immunohistochemistry. Results Ovarian abnormal, as indicated decreased E2 AMH concentrations, increased FSH concentrations. HUC-MSC caused significant upregulation downregulation (all p < 0.05). improved follicle number after transplantation. Multiple infusions 16 resulted significantly higher primordial follicles than only Week (p Immunohistochemistry that IGF-1 HGF transplantation, but this not significant. Conclusions restores disturbed secretion folliculogenesis should repeated for best treatment effect.